国产精品久久久久久亚洲毛片,欧美肥妇毛多bbwbbw,我和一个日本女生,不用下载三级黄色a影片,美国亚裔av在线观看,精品丝袜人妻久久久久久,免费三级黄色片情人,sao货真浪大ji巴cao死你,免费综合欧美高清

湖北大學(xué)張江、武玉永團(tuán)隊(duì)利用質(zhì)體轉(zhuǎn)基因技術(shù)培育了新的抗蟲(chóng)楊樹(shù)

發(fā)布時(shí)間：2019-12-06瀏覽量：11111

【字體大小：大中小】

楊樹(shù)是重要的木材資源，然而卻受到嚴(yán)重的蟲(chóng)害破壞。利用基因工程技術(shù)是解決楊樹(shù)蟲(chóng)害問(wèn)題的重要手段。我國(guó)是世界上楊樹(shù)人工林面積最大的國(guó)家，也是唯一批準(zhǔn)轉(zhuǎn)基因楊樹(shù)商業(yè)化種植的國(guó)家。最近，湖北大學(xué)會(huì)員張江、武玉永團(tuán)隊(duì)利用質(zhì)體轉(zhuǎn)基因技術(shù)培育了新的轉(zhuǎn)基因抗蟲(chóng)楊樹(shù)。

質(zhì)體轉(zhuǎn)基因具有表達(dá)量高、無(wú)位置效應(yīng)、母性遺傳等優(yōu)勢(shì)，然而尚未應(yīng)用于楊樹(shù)中。研究人員將具有鞘翅目殺蟲(chóng)活性的Bt基因cry3Bb通過(guò)基因槍導(dǎo)入楊樹(shù)質(zhì)體基因組，以防治楊樹(shù)中的主要害蟲(chóng)柳藍(lán)葉甲（Plagiodera versicolora）。cry3Bb基因表達(dá)盒還含有煙草質(zhì)體rRNA操縱子啟動(dòng)子、T7噬菌體g10（NtPrrn:T7g10）的5’-UTR和大腸桿菌核糖體RNA操縱子rrnB（TrrnB）。研究者利用Southern和Northern技術(shù)證實(shí)了外源基因的整合和表達(dá)情況，并檢測(cè)出外源Cry3Bb蛋白在轉(zhuǎn)基因楊樹(shù)嫩葉中的含量最高可達(dá)到16.8 μg/g鮮重，成熟葉片和老葉中含量有所下降。使用轉(zhuǎn)化體嫩葉喂食柳藍(lán)葉甲幼蟲(chóng)生測(cè)的結(jié)果顯示，1齡幼蟲(chóng)喂食1天后即全部死亡，2齡和3齡幼蟲(chóng)也分別在喂食2天和3天后全部死亡。

該質(zhì)體轉(zhuǎn)化技術(shù)開(kāi)發(fā)的楊樹(shù)轉(zhuǎn)化體具有很好的害蟲(chóng)防治效果。

Plant Cell Reports, 03 December 2019

Plastid-expressed Bacillus thuringiensis (Bt) cry3Bb confers high mortality to a leaf eating beetle in poplar

Author

Shijing Xu, Yiqiu Zhang, Shengchun Li, Ling Chang, Yuyong Wu, Jiang Zhang*

*: State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, China

Abstract

Poplar (Populus L.) is one of the main resource of woody industry, but being damaged by insect pests. The feasibility and efficiency of plastid transformation technology for controlling two lepidopteran caterpillars have been demonstrated previously. Here, we introduced B.thuringiensis (Bt) cry3Bb into poplar plastid genome by biolistic bombardment for controlling P.versicolora, a widely distributed forest pest. Chimeric cry3Bb geneis controlled by the tobacco plastid rRNA operon promoter combined with the 5′UTR from gene10 of bacteriophage T7 (NtPrrn:T7g10) and the 3′UTR from the E. coli ribosomal RNA operon rrnB (TrrnB). The integration of transgene and homoplasmy of transplastomic poplar plants was confirmed by Southern blot analysis. Northern blot analysis indicated that cry3Bb was transcribed to both read through and shorter length transcripts in plastid. The transplastomic poplar expressing Cry3Bb insecticidal protein showed the highest accumulation level in young leaves, which reach up to 16.8 μg/g fresh weight, and comparatively low levels in mature and old leaves. Feeding the young leaves from Bt-Cry3Bb plastid lines to P. versicolora caused 100% mortality in the first-instar larvae after only 1 day, in the second-instar larvae after 2 days, and in the third-instar larvae for 3 days. Thus, we report a successful extension of plastid engineering poplar against the chrysomelid beetle.

本文經(jīng)授權(quán)轉(zhuǎn)載自 萊肯生物公眾號(hào)